Partial Purification and Malic Enzyme Studies from Mucor circinelloides and Mortierella alpina

Role of malic enzyme during fatty acid synthesis in the oleaginous fungus Mortierella alpina.

The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in Mucor circinelloides. This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in M. circinelloides. To clarify this, the...

Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025

Mucor circinelloides produced an extracellular polygalacturonase enzyme, the production of which was enhanced when various production parameters were optimized. Maximum polygalacturonase (PGase) activity was obtained in 48 h at 30°C and pH 4.0 with pectin methyl ester (1% w/v) as carbon source and a combination of casein hydrolysate (0.1% w/v) and yeast extract (0.1% w/v) as nitrogen source. Th...

Extraction, Partial Purification and Characterization of Tannase Enzyme from Mucor sp

Introduction 1.1.20) catalyze the hydrolysis of ester and depside bonds in tannic acid [1], the reaction end products is glucose and gallic acid [2,3]. The enzyme is utilized in a number of industrial applications, is extensively used in wine, beer, coffee flavored soft drinks [4] and also including the manufacture of instant tea [5]. In addition gallic acid, finds use in the dye making, and le...

Bovine heart malic enzyme. I. Isolation and partial purification of a cytoplasmic and a mitochondrial enzyme.

Homogenates prepared from bovine heart muscle contain two distinct forms of malic enzyme (malate:NADP+ oxidoreductase (decarboxylating), EC 1.1.1.40), which can be easily separated by chromatography on DEAE-cellulose. A partial purflcation of the two enzymes has been accomplished by means of DEAE-cellulose chromatography, ammonium sulfate fractionation, and chromatography on CMSephadex. The iso...

Studies on Malic Enzyme from Hymenolepis Diminuta